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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is noticed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial function in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two additional splice variants have been claimed but detailed nucleotide sequence details just isn't however offered.

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Blocking peptides are peptides that bind specifically to your goal antibody and block antibody binding. These peptide generally incorporates the epitope recognized because of the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible success are...

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This guide outlines even more probable optimization of mobile and immunoassay parameters to be sure the absolute best success are...

Antibodies sure to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining read more with the blocked antibody compared to the antibody on your own, you can see which staining is precise.

Goal Info Cyclin E belongs into the hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinct expression and degradation designs which contribute to your temporal coordination of each mitotic party. Cyclin E kinds a posh with and features as being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is degraded as cells progress through S stage.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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